Detection of bovine respiratory syncytial virus in calves of Rio Grande do Sul, Brazil

Abstract

Durante 20 meses entre 1987 e 1990 coletou-se, em matadouro, amostras de tecido pulmonar de 351 terneiros originários de municípios próximos à Porto Alegre. Utilizando-se a imunofluorescência direta (IFD) e indireta com anticorpos policlonais e monoclonais em cortes de pulmão congelados verificou-se a presença do Vírus Respiratório Sincicial Bovino (BRSV) em 18 (5,13%) das amostras. Isolou-se o BRSV de amostras positivas pela IF.

During 20 months of the 1987-1990 period, lung tissue samples from 351 calves were obtained at a slaughterhouse. These calves were from counties nearby Porto Alegre. The direct and indirect fluorescent antibody tests (FAT) using polyclonal and monoclonal antibody conjugates were perfomed on frozen lung sections. Eighteen (5.13%) of the calf lung samples were positive for the Bovine Respiratory Syncytal Virus (BRSV). The BRSV was isolated from FAT postives samples.