On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase
dc.contributor.author | Ribeiro, Márcia Simões | pt_BR |
dc.contributor.author | Vasconcellos Júnior, Fábio Jorge de | pt_BR |
dc.contributor.author | Fonseca, Bruna Teixeira da | pt_BR |
dc.contributor.author | Souza, Flávia Carvalho de | pt_BR |
dc.contributor.author | Soares, Felipo Doval Rojas | pt_BR |
dc.contributor.author | Lima, Éder Cláudio | pt_BR |
dc.contributor.author | Cabral, Murilo Feitosa | pt_BR |
dc.contributor.author | Ribeiro, Emerson Schwingel | pt_BR |
dc.contributor.author | D'Elia, Eliane | pt_BR |
dc.date.accessioned | 2014-01-16T01:54:45Z | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.issn | 1759-9660 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/85929 | pt_BR |
dc.description.abstract | In the present work, two mixed oxides, namely, SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 (designated as SiAlNb and SiAlTi, respectively), obtained using the sol–gel method were used to immobilize chloroperoxidase. Hydrogen peroxide was quantified using potassium hexacyanoferrate(II) as a redoxmediator and amperometric measurements at 0.0 V vs. Ag/AgCl/Cl (3 M). The SiAlTi biosensor presented higher sensitivity than the SiAlNb biosensor, however, the first one did not present a good response regarding time. The developed biosensor using the SiAlNb mixed oxide provided good signal levels, good linearity, good stability (retaining approximately 70% of its original response after 6 weeks of usage), a low detection limit (3 цM), good sensitivity, a suitable working range (from 4 to 19 цM), fast response and good repeatability. The recovery of the amperometric method for the detection of hydrogen peroxide in synthetic samples was approximately 100 ±2%, and for Listerine® Whitening Pre- Brush Rinse samples fortified with 1, 2 and 3% (v/v) of hydrogen peroxide, it was 100 ±3%. | en |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Analytical Methods. Londres. Vol. 6, no. 2 (Jan. 2014), p. 521-528 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Química analítica | pt_BR |
dc.subject | Sol-gel | pt_BR |
dc.subject | Biosensores | pt_BR |
dc.title | On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000909143 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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