2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells
dc.contributor.author | Volkmer, Tiago Moreno | pt_BR |
dc.contributor.author | Magalhães, Joana Cristina Silva | pt_BR |
dc.contributor.author | Sousa, Vânia Caldas de | pt_BR |
dc.contributor.author | Santos, Luis Alberto dos | pt_BR |
dc.contributor.author | Burguera, Elena Fernández | pt_BR |
dc.contributor.author | Blanco Garcíaz, Francisco Javier | pt_BR |
dc.contributor.author | San Román, Julio | pt_BR |
dc.contributor.author | Rodríguez-Lorenzo, Luis M. | pt_BR |
dc.date.accessioned | 2023-11-25T03:26:19Z | pt_BR |
dc.date.issued | 2014 | pt_BR |
dc.identifier.issn | 2073-4360 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/267620 | pt_BR |
dc.description.abstract | The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Polymers [recurso eletrônico]. Basel. Vol. 6 (2014), p. 2510-2525 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Cryogels | en |
dc.subject | Biomateriais | pt_BR |
dc.subject | Engenharia de tecidos | pt_BR |
dc.subject | α-tricalcium phosphate | en |
dc.subject | Biopolímeros | pt_BR |
dc.subject | Mesenchymal stem cells | en |
dc.title | 2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cells | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 000949609 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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