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dc.contributor.authorVolkmer, Tiago Morenopt_BR
dc.contributor.authorMagalhães, Joana Cristina Silvapt_BR
dc.contributor.authorSousa, Vânia Caldas dept_BR
dc.contributor.authorSantos, Luis Alberto dospt_BR
dc.contributor.authorBurguera, Elena Fernándezpt_BR
dc.contributor.authorBlanco Garcíaz, Francisco Javierpt_BR
dc.contributor.authorSan Román, Juliopt_BR
dc.contributor.authorRodríguez-Lorenzo, Luis M.pt_BR
dc.date.accessioned2023-11-25T03:26:19Zpt_BR
dc.date.issued2014pt_BR
dc.identifier.issn2073-4360pt_BR
dc.identifier.urihttp://hdl.handle.net/10183/267620pt_BR
dc.description.abstractThe aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the α-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% ± 5.7% for a greater content in PHEMA and 75% ± 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The α-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.en
dc.format.mimetypeapplication/pdfpt_BR
dc.language.isoengpt_BR
dc.relation.ispartofPolymers [recurso eletrônico]. Basel. Vol. 6 (2014), p. 2510-2525pt_BR
dc.rightsOpen Accessen
dc.subjectCryogelsen
dc.subjectBiomateriaispt_BR
dc.subjectEngenharia tecidualpt_BR
dc.subjectα-tricalcium phosphateen
dc.subjectBiopolímerospt_BR
dc.subjectMesenchymal stem cellsen
dc.title2-(Dimethylamino)ethyl methacrylate/(2-hydroxyethyl) methacrylate/α-tricalcium phosphate cryogels for bone repair, preparation and evaluation of the biological response of human trabecular bone-derived cells and mesenchymal stem cellspt_BR
dc.typeArtigo de periódicopt_BR
dc.identifier.nrb000949609pt_BR
dc.type.originEstrangeiropt_BR


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