Differential immunomodulatory effects of head and neck cancer-derived exosomes on B cells in the presence of ATP
dc.contributor.author | Coray, Mali | pt_BR |
dc.contributor.author | Göldi, Veronika | pt_BR |
dc.contributor.author | Schmid, Lukas | pt_BR |
dc.contributor.author | Benecke, Laura | pt_BR |
dc.contributor.author | Figueiró, Fabrício | pt_BR |
dc.contributor.author | Muller, Laurent | pt_BR |
dc.date.accessioned | 2022-12-09T04:58:56Z | pt_BR |
dc.date.issued | 2022 | pt_BR |
dc.identifier.issn | 1422-0067 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/252577 | pt_BR |
dc.description.abstract | Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy. Tumor-derived exosomes (TEX) have immunoregulatory properties. Adenosine triphosphate (ATP) and its immunosuppressive precursor adenosine (ADO) have been found in cancerous tissue. We investigated the effect of TEX on B cells in the presence of ATP. TEX were isolated from human HNSCC cell line (PCI-13) cultures and co-cultured with peripheral blood B cells of healthy donors, with or without TEX in different concentrations and with or without a low (20 µM) or high (2000 µM) ATP dose. We were able to demonstrate that TEX inhibit B-cell proliferation. The addition of TEX to either ATP concentration showed a decreasing trend in CD39 expression on B cells in a dose-dependent manner. High ATP levels (2000 µM) increased apoptosis and necrosis, and analysis of apoptosis-associated proteins revealed dose-dependent effects of ATP, which were modified by TEX. Altogether, TEX exhibited dual immunomodulatory effects on B cells. TEX were immunosuppressive by inhibiting B-cell proliferation; they were immunostimulatory by downregulating CD39 expression. Furthermore, TEX were able to modulate the expression of pro- and anti-apoptotic proteins. In conclusion, our data indicate that TEX play an important, but complex, role in the tumor microenvironment. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | International journal of molecular sciences. Basel. Vol. 23, no. 22 (Nov. 2022), 14446, 21 p. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Neoplasias de cabeça e pescoço | pt_BR |
dc.subject | Tumor-derived exosomes | en |
dc.subject | HNSCC | en |
dc.subject | Exossomos | pt_BR |
dc.subject | Exosomes | en |
dc.subject | Trifosfato de adenosina | pt_BR |
dc.subject | Extracellular vesicles | en |
dc.subject | Carcinoma de células escamosas de cabeça e pescoço | pt_BR |
dc.subject | B cells | en |
dc.subject | Imunomodulacao | pt_BR |
dc.subject | Regulatory B cells | en |
dc.subject | ATP | en |
dc.subject | Adenosine | en |
dc.subject | Immunomodulation | en |
dc.subject | Apoptosis | en |
dc.title | Differential immunomodulatory effects of head and neck cancer-derived exosomes on B cells in the presence of ATP | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001155243 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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