Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition
dc.contributor.author | Puukila, Stephanie | pt_BR |
dc.contributor.author | Bryan, Sean | pt_BR |
dc.contributor.author | Laakso, Anna | pt_BR |
dc.contributor.author | Abdel-Malak, Jessica | pt_BR |
dc.contributor.author | Gurney, Carli | pt_BR |
dc.contributor.author | Agostino, Adrian | pt_BR |
dc.contributor.author | Belló-Klein, Adriane | pt_BR |
dc.contributor.author | Prasad, Kailash | pt_BR |
dc.contributor.author | Khaper, Neelam | pt_BR |
dc.date.accessioned | 2021-07-29T04:31:23Z | pt_BR |
dc.date.issued | 2015 | pt_BR |
dc.identifier.issn | 1932-6203 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/224774 | pt_BR |
dc.description.abstract | Cardiac iron overload is directly associated with cardiac dysfunction and can ultimately lead to heart failure. This study examined the effect of secoisolariciresinol diglucoside (SDG), a component of flaxseed, on iron overload induced cardiac damage by evaluating oxidative stress, inflammation and apoptosis in H9c2 cardiomyocytes. Cells were incubated with 50 μ5M iron for 24 hours and/or a 24 hour pre-treatment of 500 μ M SDG. Cardiac iron overload resulted in increased oxidative stress and gene expression of the inflammatory mediators tumor necrosis factor-α, interleukin-10 and interferon γ, as well as matrix metalloproteinases-2 and -9. Increased apoptosis was evident by increased active caspase 3/7 activity and increased protein expression of Forkhead box O3a, caspase 3 and Bax. Cardiac iron overload also resulted in increased protein expression of p70S6 Kinase 1 and decreased expression of AMP-activated protein kinase. Pre-treatment with SDG abrogated the iron-induced increases in oxidative stress, inflammation and apoptosis, as well as the increased p70S6 Kinase 1 and decreased AMP-activated protein kinase expression. The decrease in superoxide dismutase activity by iron treatment was prevented by pre-treatment with SDG in the presence of iron. Based on these findings we conclude that SDG was cytoprotective in an in vitro model of iron overload induced redox-inflammatory damage, suggesting a novel potential role for SDG in cardiac iron overload. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | PloS one. San Francisco. Vol. 10, no. 3 (Mar. 2015), e0122852, 16 f. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Sobrecarga de ferro | pt_BR |
dc.subject | Estresse oxidativo | pt_BR |
dc.subject | Cardiopatias | pt_BR |
dc.title | Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001058162 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
Este item está licenciado na Creative Commons License
![](/themes/Mirage2Novo//images/lume/cc.png)
-
Artigos de Periódicos (39351)Ciências Biológicas (3068)