Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes
dc.contributor.author | Gonçalves, Fabiany da Costa | pt_BR |
dc.contributor.author | Luk, Franka | pt_BR |
dc.contributor.author | Korevaar, Sander S. | pt_BR |
dc.contributor.author | Bouzid, Rachid | pt_BR |
dc.contributor.author | Paz, Ana Helena da Rosa | pt_BR |
dc.contributor.author | Iglesias, Carmen López | pt_BR |
dc.contributor.author | Baan, Carla C. | pt_BR |
dc.contributor.author | Merino, Ana | pt_BR |
dc.contributor.author | Hoogduijn, Martin J. | pt_BR |
dc.date.accessioned | 2018-04-26T02:33:33Z | pt_BR |
dc.date.issued | 2017 | pt_BR |
dc.identifier.issn | 2045-2322 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/175085 | pt_BR |
dc.description.abstract | Mesenchymal stromal cells (MSC) are a promising therapy for immunological disorders. However, culture expanded MSC are large and get trapped in the capillary networks of the lungs after intravenous infusion, where they have a short survival time. Hypothetically, living cells are a risk for tumor formation. To reduce risks associated with MSC infusion and improve the distribution in the body, we generated membrane particles (MP) of MSC and MSC stimulated with IFN-γ (MPγ). Tracking analysis and electron microscopy indicated that the average size of MP was 120 nm, and they showed a round shape. MP exhibited ATPase, nucleotidase and esterase activity, indicating they are enzymatically active. MP and MPγ did not physically interact with T cells and had no effect on CD4+ and CD8+ T cells proliferation. However, MP and MPγ selectively bound to monocytes and decreased the frequency of pro-inflammatory CD14+CD16+ monocytes by induction of selective apoptosis. MP and MPγ increased the percentage of CD90 positive monocytes, and MPγ but not MP increased the percentage of antiinflammatory PD-L1 monocytes. MPγ increased mRNA expression of PD-L1 in monocytes. These data demonstrate that MP have immunomodulatory properties and have potential as a novel cell-free therapy for treatment of immunological disorders. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Scientific reports. London. Vol. 7 (Sep. 2017), 12100, 13 f. | pt_BR |
dc.rights | Open Access | en |
dc.subject | Células mesenquimais estromais | pt_BR |
dc.subject | Immunosuppression | en |
dc.subject | Imunidade | pt_BR |
dc.subject | Mesenchymal stem cells | en |
dc.subject | Tecido adiposo | pt_BR |
dc.title | Membrane particles generated from mesenchymal stromal cells modulate immune responses by selective targeting of pro-inflammatory monocytes | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001065696 | pt_BR |
dc.type.origin | Estrangeiro | pt_BR |
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