Effect of soybean ureases on seed germination and plant development
dc.contributor.author | Rechenmacher, Ciliana | pt_BR |
dc.contributor.author | Strohm, Beatriz Wiebke | pt_BR |
dc.contributor.author | Busatto, Luisa Abruzzi de Oliveira | pt_BR |
dc.contributor.author | Polacco, Joseph Carmine | pt_BR |
dc.contributor.author | Carlini, Celia Regina Ribeiro da Silva | pt_BR |
dc.contributor.author | Bodanese-Zanettini, Maria Helena | pt_BR |
dc.date.accessioned | 2017-09-05T02:32:35Z | pt_BR |
dc.date.issued | 2017 | pt_BR |
dc.identifier.issn | 1415-4757 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/10183/166137 | pt_BR |
dc.description.abstract | Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5- co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes, as well as a urease-null mutant (eu3-a) that activates neither the ubiquitous nor embryo-specific ureases. The co-suppressed plants presented a developmental delay during the first month after germination; shoots and roots were significantly smaller and lighter. Slower development was observed for the double eu1-a/eu4-a mutant and the eu3-a single mutant. The N content in transgenic plants was significantly lower than in non-transgenic plants. Among the mutants, eu3-a presented the lowest and eu1-a the highest N content. Altogether, these results indicate that increased ureolytic activity plays an important role in plant development. | en |
dc.format.mimetype | application/pdf | pt_BR |
dc.language.iso | eng | pt_BR |
dc.relation.ispartof | Genetics and molecular biology. Ribeirão Preto, SP. Vol. 40, no. 1, suppl. 1 (2017), p. 209-216 | pt_BR |
dc.rights | Open Access | en |
dc.subject | Desenvolvimento vegetal | pt_BR |
dc.subject | Plant development | en |
dc.subject | Plantas transgênicas | pt_BR |
dc.subject | Functional study | en |
dc.subject | Transgenic plants | en |
dc.subject | Urease | pt_BR |
dc.subject | Soja | pt_BR |
dc.subject | Mutants | en |
dc.subject | Urease | en |
dc.title | Effect of soybean ureases on seed germination and plant development | pt_BR |
dc.type | Artigo de periódico | pt_BR |
dc.identifier.nrb | 001026729 | pt_BR |
dc.type.origin | Nacional | pt_BR |
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